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. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Nat Chem Biol. 2022 Mar 14;18(5):511–519. doi: 10.1038/s41589-022-00981-0

Fig. 4 |. Receptor binding affinity.

Fig. 4 |

a, Competition binding assays of human insulin and Vh-Ins-HALQ to solubilized and immobilized insulin receptor (IR-B). Note that IR-B generally binds insulin and analogs with similar affinity to IR-A33. Each data point represents the mean of three assays. Error bars (s.e.m.) are shown. b, Type 1 IGF receptor competition binding assay of human insulin, hIGF-I and Vh-Ins-HALQ. Each data point represents the mean of two assays (each with three technical replicates). Error bars (s.e.m.) are shown. Results in a and b are expressed as a percentage of binding in the absence of competing ligand.