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. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Nat Chem Biol. 2022 Mar 14;18(5):511–519. doi: 10.1038/s41589-022-00981-0

Extended Data Fig. 2. AKT phosphorylation activity of Vh-Ins-HTLQ and related analogs.

Extended Data Fig. 2

NIH 3T3 cells overexpressing IR-B were stimulated with insulin analogs and pAkt was quantified using a homogeneous time-resolved fluorescence assay. Error bars (s.e.m. of 4 biological replicates) are shown when larger than the symbols. Two substitutions on the B chain, GluB10 and LeuB20, were found to increase the relative activity of Vh-Ins-HTLQ. These substitutions were subsequently included in later stages of design of Vh-Ins molecules.