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. 2022 Jun 17;12:881022. doi: 10.3389/fonc.2022.881022

Figure 2.

Figure 2

ELFN1-AS1 promoted the proliferation, migration and invasion of OS cells. (A) RT-qPCR analysis of ELFN1-AS1 level in 143B and MG63 cells transfected with ELFN1-AS1 siRNA1 or ELFN1-AS1 siRNA2 (n = 3). (B) RT-qPCR analysis of ELFN1-AS1 level in 143B and MG63 cells transfected with pcDNA3.1 ELFN1-AS1 (n = 3). (C, D) 143B and MG63 cells were transfected with pcDNA3.1 ELFN1-AS1 or ELFN1-AS1 siRNA1. CCK-8 assay was applied to measure cell viability (n = 3). (E) EdU staining assay was applied to detect cell proliferation (n = 3). (F) Flow cytometry assay was applied to determine cell apoptosis. (G) Transwell migration and (H) invasion assays were applied to determine cell migration and invasion (n = 3). The significance between groups was analyzed by one-way ANOVA. **P < 0.01.