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. 2022 Jun 17;12:881022. doi: 10.3389/fonc.2022.881022

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Copyright © 2022 Wang, Wang, Li, Niu, Liang, Liu, Liu and Ge

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exosomes with upregulated ELFN1-AS1 promoted macrophages M2 polarization. (A) Exosomes were isolated from the CM of 143B cells transfected with pcDNA3.1 ELFN1-AS1 (143B/pcDNA3.1-ELFN1-AS1-Exo) or ELFN1-AS1 siRNA1 (143B/ELFN1-AS1 siRNA1-Exo). The size of exosomes was assessed by NTA assay. The morphology of exosomes was observed by TEM at 100 keV. Red arrow, exosomes. (B) The expression of exosome surface markers CD81 and TSG101 were detected with western blot assay (n = 3). (C) RT-qPCR analysis of ELFN1-AS1 level in macrophages (n = 3). (D, E) RT-qPCR analysis of CD206 and CD163 level in macrophages (n = 3). The significance between groups was analyzed by one-way ANOVA. *P < 0.05; **P < 0.01.