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. 2022 Jun 17;12:881022. doi: 10.3389/fonc.2022.881022

Figure 7.

Figure 7

Exosomal ELFN1-AS1 promoted macrophage M2 polarization via regulating miR-1291/CREB1 axis. (A) Schematic diagram of binding sites between ELFN1-AS1 and miR-1291. (B) RT-qPCR analysis of miR-1291 level in macrophages after transfecting with miR-1291 mimics and miR-1291 inhibitor (n = 3). (C) Luciferase activity in macrophages treated with the ELFN1-AS1-wt/mut plasmids together with miR-1291 mimics was analyzed (n = 3). (D) FISH detection of ELFN1-AS1 and miR-1291 in macrophages. (E) RNA pull-down assays were used to determine the interaction between ELFN1-AS1 and miR-1291 (n = 3). (F) Schematic diagram of binding sites between CREB1 and miR-1291. (G) Luciferase activity in macrophages treated with the CREB1-wt/mut plasmids together with miR-1291 mimics was analyzed (n = 3). (H) RT-qPCR analysis of CREB1 level in transfected macrophages (n = 3). (I) Western blot analysis of CREB1 expression in transfected macrophages (n = 3). (J) RT-qPCR analysis of CD206, IL-10, iNOS level in transfected macrophages (n = 3). The significance between two or more groups was analyzed by Student’s t test or one-way ANOVA respectively. **P < 0.01.