Figure 2.

Keratinocyte-derived sEVs are enriched in glycoproteins involved in adhesion in steady state and in AD milieu. (A) Enrichment of adhesion proteins involved in cell adhesion in sEV_NHEK secreted in steady-state keratinocytes; Reactome - Gene Ontology terms re-analysis of the data available from Chavez-Muñoz et al. (28); (B) enrichment of adhesion proteins involved in cell adhesion in sEV_NHEK secreted by NHEKs exposed to AD milieu as identified by Reactome - Gene Ontology terms analysis; (C) interaction network for sEV adhesion-relevant proteins identified in sEV_0.06NHEK and sEV_1.5NHEK; (D) cell adhesion-relevant glycoproteins identified by mass spec in sEV_0.06NHEK and sEV_1.5NHEK; (E) Reactome-Gene Ontology identified term enrichment for the proteins in sEV_0.06NHEK and sEV_1.5NHEK; FDR, False Discovery Rate; N.B. classical exosomal glycoprotein markers are included in the supplementary data ( Figure S2D ); mass spectrometry data based on n=3 biological replicates.