Figure 6.

Sialyltransferase ST6GAL1 is implicated in sialylation pattern on keratinocyte-derived sEVs and defines their cell interaction propensity via Siglec-7 and Siglec-9 receptors. (A) Identification of binding receptors for sEV_N/TERT-1 cell interaction by blocking experiments with anti Siglec-type receptors on differentiated THP-1 cells; combined data for n=3 biological replicates; (B) single-cell RNA expression levels of Siglec-7 and Siglec-9 in the epidermis of the skin; data available through ProteinAtlas; (C) UMAP plots of single cell expression of SIGLEC7 and SIGLEC9 receptors in the skin; data avalabale through Human Developmental Cell Atlas; (D) protein interaction network of ST6GAL1 and C1GALT1 enzymes obtained via STRING analysis. * - p<0.05, *** - p<0.001.