Fig. 4.

Der p 2 triple mutant (D59K, L61K, and K100D) allergen does not induce anaphylaxis in hFcεRI mice. Human FcεRIα–transgenic mice were sensitized with 100 µg total of human Der p 2-specific IgE mAbs 2F10 + 2G1 or 4C8 + 2G1. Animals were challenged with 50 µg of recombinant Der p 2 wildtype or triple mutant (D59K, L61K, and K100D). Anaphylaxis was monitored using an implanted temperature probe for 90 min following challenge. Time points with calculated P-values < 0.05 are underscored with a colored bar. Data are means ± SD of each experimental group. The number of mice (n) for each experimental group is shown.