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. 2022 Jun 28;39(13-14):979–998. doi: 10.1089/neu.2022.0012

FIG. 8.

FIG. 8.

Glucocorticoid receptor (GR) overexpression in the dorsal hippocampus facilitates GR nuclear translocation in brain-injured animals. The effect of viral transfection of human GR (hGR) on GR protein levels in the dorsal hippocampus was measured using Western blot in dorsal hippocampal samples harvested immediately following the 1st day of water maze training. Representative blots demonstrating GR and actin bands in the cytoplasmic fraction (n = 6–8/group) are shown in A; representative blots demonstrating GR and lamin bands in the nuclear fraction (n = 6–8/group) are shown in B. (C) The signal of the GR band (normalized to actin signal and displayed as percent of sham control expression) in the cytoplasmic fraction. (D) The signal of the GR band (normalized to lamin signal and displayed as percent of sham control expression) in the nuclear fraction. (E) The ratio of normalized GR signal in the nuclear fraction relative to the cytoplasmic fraction (displayed as percent of sham control expression). Error bars represent standard error of the mean. *p < 0.05. NF, nuclear fraction; CF, cytoplasmic fraction. Color image is available online.