TABLE 1.
Specificity of fluorogenic PCR to detect Salmonella and E. coli O157:H7
| Bacterial strainsa | Serotype, species, or groupb | eae gene presentc | sipB-sipC genes presentc | Fluorogenic PCR detection
|
|
|---|---|---|---|---|---|
| E. coli O157:H7 specific | Salmonella specific | ||||
| Nontoxigenic E. coli | O111:H12 (2) | − | − | − | − |
| H21 (3) | − | − | − | − | |
| O149:HN | − | − | − | − | |
| O157:H43 | − | − | − | − | |
| O128:H7 (2) | − | − | − | − | |
| H21 | − | − | − | − | |
| H47 | − | − | − | − | |
| ETEC | O101 | − | − | − | − |
| O149:H10 | − | − | − | − | |
| RDEC | O132 | + | − | − | − |
| O15:HN | + | − | − | − | |
| EPEC | O55:NM (2) | + | − | − | − |
| H6 (2) | + | − | − | − | |
| O26:NM (3) | + | − | − | − | |
| O111:H2 (3) | + | − | − | − | |
| O128:H2 (4) | + | − | − | − | |
| O45:H2 | + | − | − | − | |
| O55:H7 (2) | + | − | + | − | |
| STEC | O111:NM (2) | + | − | − | − |
| O111:H8 | + | − | − | − | |
| O26:H11 | + | − | − | − | |
| O45:H2 | − | − | − | − | |
| O113 | − | − | − | − | |
| OX3:H2 | − | − | − | − | |
| EHEC | O26:H11 (3) | + | − | − | − |
| O111:NM | + | − | − | − | |
| O157:H7 (5) | + | − | + | − | |
| O157:NM (1) | + | − | + | − | |
| Salmonella | Typhimurium | − | + | − | + |
| Enteriditis | − | + | − | + | |
| Samftenberg | − | + | − | + | |
| Litchfield | − | + | − | + | |
| DT104 (2) | − | + | − | + | |
| B1RC8 | − | + | − | + | |
| B1RC9 | − | + | − | + | |
| EILC9 | − | + | − | + | |
| E1LC10 | − | + | − | + | |
| C1CC117 | − | + | − | + | |
| Enterobacter | E. cloacae | − | − | − | − |
| Hafnia | H. alvie | + | − | − | − |
| Klebsiella | K. pneumoniae | − | − | − | − |
| Proteus | P. vulgaris | − | − | − | − |
| Pseudomonas | P. aeruginosa | − | − | − | − |
| Staphylococcus | S. aureus | − | − | − | − |
a
ETEC, enterotoxigenic E. coli; RDEC, rabbit diarrheagenic E. coli; EPEC, enteropathogenic E. coli; STEC, Shiga toxin-producing E. coli; EHEC, enterohemorrhagic E. coli.
b
Parentheses indicate the number of isolates of a serotype tested.
c
The presence of the eae gene in E. coli and that of sipBC in Salmonella was determined by colony blot hybridization using probes specific for these genes (2, 11; http://www.bio.psu.edu/people/faculty/whittam/lab/deca/).