Fig. 3.

GSCs are enriched in recurrent xenografts and patient GBMs. (A) Relative mRNA expression levels of 28 GSC markers in primary (n = 3) vs recurrent (n = 4) pooled xenografts. (B–D) Representative images of SOX2, OLIG2, and POU3F2 immunofluorescence staining and software-based quantification in primary (n = 9) vs recurrent (n = 17) xenografts. Scale = 50 µm (overview) and 10 µm (inserts). (E–G) Validation of SOX2, OLIG2, and POU3F2 protein upregulation in primary (n = 11) vs early recurrent (n = 11) patient GBMs. Scale = 50 µm (overview) and 10 µm (inserts). AU = arbitrary units. Abbreviations: GBMs, glioblastomas; GSCs, GBM stem cells.