Figure 5.

Thrombin-mediated effects on fluid phase-dependent complement activation. (A) Apical supernatants of treated cells were analyzed for thrombin-mediated complement component C3 (left blot) and C5 (right-hand blot) cleavage, using antibodies against C3a and C5a respectively. (B) Reduction of the 120 kDa and generation of an 80 kDa fragment of C3 are assessed by densitometric analysis using image J. (C) Reduction of the 120 kDa band could be inhibited by dabigatran (10 μM) and the fluid phase complement inhibitor compstatin (100 μM) (left hand blot) but not the inhibitor of the alternative pathway TT30 (10 μM) that requires membrane binding (right hand blot). Data are plotted as mean ± SEM; n = 3 independent experiments.