| Antibodies |
|
|
|
| anti-Astrocyte cell surface antigen 2 (ACSA-2) |
Miltenyi Biotec |
130-097-678; RRID:AB_2894998 |
| anti-CD11b |
Miltenyi Biotec |
130-126-725 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Deferiprone |
ApoPharma |
N/A |
| Recombinant human IL-1β protein |
Abcam |
ab9617 |
| Recombinant mouse IL-1β protein |
LSBio |
LS-G51411
|
|
| Critical commercial assays |
|
|
|
| MDA ELISA |
Abcam |
ab238537 |
| Mouse IL-1β ELISA |
R&D Systems |
MLB00C |
| Human IL-1β ELISA |
R&D Systems |
DLB50 |
|
| Deposited data |
|
|
|
| Human choroidal scRNA-seq data |
Voigt et al. (2019)
|
GSE135922 donors 4–7 (control), 22–23 (AMD) |
| Neurosensory retina scRNA-seq data |
Lyu et al. (2021)
|
GSE155288 |
| Neurosensory retina bulk RNA-seq data |
Lyu et al. (2021)
|
GSE155154 |
|
| Experimental models: Organisms/strains |
|
|
|
| C57BL6/J Wild-Type |
Jackson Labs |
000664 |
| IL-1β Knockout |
Dr. Iwakura, University of Tokyo |
N/A |
| Experimental models: Cell culture |
|
|
| Fetal RPE Cell Cultures |
Advanced Bioscience Resources Inc. |
N/A |
|
| Software and algorithms |
|
|
|
| Seurat R package |
Butler et al. (2018)
|
https://satijalab.org/seurat/
|
|
| Other |
|
|
|
| Rodent diet with 10% fat (low fat diet) |
Research Diets |
D12450 |
| Rodent diet with 60% fat (high fat diet) |
Research Diets |
D12492 |
