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. 2022 Jun 17;13:865424. doi: 10.3389/fimmu.2022.865424

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Copyright © 2022 Wagner, Koehl, Chmielewski, Scheid and Stripecke

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison between retroviral vector and lentiviral vector (RV/LV) gene delivery systems with CRISPR-Cas gene editing for production of chimeric antigen receptor (CAR)-T cells. (A) Scheme of T cell transduction with RV/LV (left) and cell transfection with ribonucleoprotein (RNP, Right). (B) Schematic representation of genetic structures. Upper structure: Displays an integrated prototypic LV gene transfer vector encoding a CAR, not to scale. LTR: Long terminal repeats; HIV: Human immune deficient virus U5: Untranslated region in the 5’ side; Ψ: encapsidation signal; RRE, Rev responsive element; cPPT, polypurine tract; EF1α, Elongation factor 1 α. Lower structure: Represents a prototypic integrated CAR generated by gene editing. TRAC, Locus of T cell receptor alpha chain; HDR, Homology-directed recombination.