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. 2022 Jun 17;13:937910. doi: 10.3389/fmicb.2022.937910

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Copyright © 2022 Dumoulin, Vollrath, Won, Wang and Burleigh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Measurement of epimastigote membrane integrity by flow cytometry using propidium iodide staining following incubation for 4 h with the indicated concentrations of amphotericin B (n = 3), mean ± SD shown. (B) Measurement of epimastigote concentration every 48 h starting at 1e5 epimastigotes/ml (n = 3), mean ± SD shown. (C) Growth and (D) membrane integrity of epimastigotes assessed daily during continuous exposure to either 27°C or 37°C (n = 3), mean ± SD shown. (E) (i) Flow cytometry histograms of parasite fluorescence in the Y2 (615/20) channel. Unstained controls are shown along with TMRE stained parasites. (ii) Mean fluorescence intensity (MFI) in the Y2 channel with and without pre-treatment of parasites with FCCP ±SD shown. Parasite line, FCCP treatment and interaction have p < 0.001 by two-way ANOVA; all multiple pairwise comparisons compared to WT are significant after Bonferroni correction.