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. 2022 May 5;80(1):95–109. doi: 10.1097/FJC.0000000000001271

FIGURE 3.

FIGURE 3.

MiR-503 inhibited PASMC migration and proliferation by inhibiting the PI3K/AKT axis. A, The potential target sites of miR-503 matching the 3ʹ-UTR of PI3K. B, Compared with the negative control (WT + negative control), when miR-503 was added to wild-type (WT) PI3K, it inhibited luciferase activity, whereas miR-503 had no effect on mutant PI3K (n = 6). C, CCK-8 experiment (n = 6). (D), (G), Cell migration was determined by transwell assays at 24 hours (n = 3) (×100). E, Immunoblotting analysis of PI3K expressions in PASMCs of each group (n =3). (F), (H), EdU incorporation experiment (n = 3) were used to evaluate cell viability (×200). (I), (J), Immunoblotting analysis of the expression of PI3K, AKT, p-AKT and PCNA in PASMCs from each treatment group (n = 3).