FIGURE 8.

The therapeutic effect of ELAVL2 in PH acted through miR-503. A, miR-503 expression in rat PASMCs to which ELAVL2 siRNA or control siRNA was transfected was measured by qRT-PCR (n = 3). B, An RBP immunoprecipitation assay was performed to investigate the binding of ELAVL2 to pre-miRNA-503. Results were quantified by qRT-PCR (n = 3). (C), (D), Cell migration was determined by transwell assays at 24 hours (n = 3) (×100). (E), (F), EdU incorporation experiment (n = 3) were used to evaluate cell viability (×200). OE means overexpression of ELAVL2.