Figure 1. Differentially abundant gut taxa among children aged 6–61 months who were cluster randomized to an automated chlorinated water intervention in urban Bangladesh and effects on overall richness and diversity.

Panel A) is a differential heat tree depicting the taxonomies of bacterial genera and families that significantly differed in their relative abundance between treatment and control children. Genera within differentially abundant families are also depicted. For any given taxonomic level, only taxa that were significantly less abundant (orange) or more abundant (purple) among treatment relative to control children are depicted in color; non-significant taxa are depicted in gray. Panel B) depicts estimated genera richness and Panel C) depicts Shannon diversity indices for treatment and control children, stratified by child age. Estimated genera richness differed between treatment and control children aged 15–30 months by two-sided Wilcoxon signed-rank test (p=0.04); Shannon diversity did not significantly differ for any age stratum using two-sided Wilcoxon signed-rank tests. For all box plots, center line indicates the median; box limits indicate the upper and lower quartiles; and whiskers indicate 1.5x interquartile range. Panels D) – E) are differential heat trees depicting the taxonomies of bacterial genera and families that significantly differed in their relative abundance between treatment and control children aged D) 6–14 months, E) 15–30 months, and F) 31–61 months, controlling for study site.

Note: ns=non-significant. *indicates p<0.05 by Wilcoxon signed-rank test.