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Donor substrate specificity of MBP-Δ27hST3GAL II-His6.
| Donor precursor | CMP-sialic acid yield (%) | Sialoside yield | |
|---|---|---|---|
| 20 min (%) | 16 h (%) | ||
| Neu5Ac | 100 ±1.2 | 62.6 ±1.2 | 100 ±0 |
| Kdn | 91.2 ±1.6 | 41.8 ±2.5 | 87.6 ±2.3 |
| ManNAc | 89.9 ±0.1 | 53.1 ±2.4 | 98.3 ±2.4 |
| Mannose | 86.3 ±2.1 | 40.8 ±3.8 | 86.0 ±0.8 |
| Man2N3 | 78.3 ±4.5 | 28.9 ±1.1 | 46.3 ±5.5 |
| ManNAc | 96.9 ±3.1 | 62.3 ±0.4 | 100 ±0.0 |
| ManNAc4N3 | 85.3 ±2.8 | 27.5 ±2.4 | 64.0 ±1.9 |
| ManNAc6N3 | 90.3 ±1.2 | 48.1 ±1.4 | 95.3 1.2 |
