Table 3.

Percentage (%) of sialoside Neu5Acα2–3Galβ1–3GalNAcαProNHCbz (13) (the product of the sialyltransferase activity) that is desialylated by different amounts of MBP-Δ27hST3GAL II-His₆ in the presence of CMP for 1 h or 20 h.

Reaction Time	190 μU[a]	19 μU[a]	1.9 μU[a]
1 h	1.4±0.1	0.2±0.3	ND[b]
20 h	3.9±0.1	2.3±0.1	0.6±0.1

^[a]The amounts of enzyme used are shown in units for its sialyltransferase activity: 1 unit of the sialyltransferase activity is defined as the amount of the enzyme that catalyzes the formation of 1 μmol of sialylated product Neu5Acα2–3Galβ1–3GalNAcαProNHCbz from Galβ1–3GalNAcαProNHCbz at 37 °C per minute at pH 7.0.

^[b]ND, not detected.