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Normalized plot of toluene-d8 and benzylsuccinate-d8 concentration in a methanogenic enrichment culture. Toluene concentration is normalized to the total consumed (∼500 μM), and labeled benzylsuccinate is normalized to its maximum observed concentration (∼0.01 μM). Benzylsuccinate-d8 was quantified by liquid chromatography-MS-MS, and toluene-d8 was quantified by GC-flame ionization detection.
