|
| ||
| Component | Volume | |
|
| ||
| 2X HiFi ReadyMix | 20 μL | 20 μL |
|
| ||
| 10 μM FWD | 1.2 μL | 2.0 μL |
|
| ||
| 10 μM pX330-REV | 1.2 μL | - |
|
| ||
| 10 μM sgRNA-REV | - | 2.0 μL |
|
| ||
| DNAse/RNAse-free H2O | 13.6 μL | 16 μL |
|
| ||
| 1 ng/μL pX330 | 4 μL | - |
|
| ||
| TOTAL | 4 μL | 4 μL |
|
| ||
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