|
| ||
| Component | Final Concentration - pronuclear | Final Concentration - cytoplasmic |
|
| ||
| Cas9 mRNA | 30 ng/μL | 100 ng/μL |
|
| ||
| gRNA | 10 ng/μL | 10 ng/μL |
|
| ||
| Repair template | 10 ng/μL (~0.25 μM) | 100 ng/μL (~2.5 μM) |
|
| ||
| Microinjection buffer | to 75 μL | to 75 μL |
|
| ||
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