|
| ||
| Component | Final concentration | Notes |
|
| ||
| Nuclease-free water | to 11 μL for cuvette electroporation; to 5 μL for slide electroporation | a |
|
| ||
| 5X Cas9 buffer | 1X | |
|
| ||
| gRNA(s) | 8.8 μM | b |
|
| ||
| 61 μM Cas9 protein | 8 μM | |
|
| ||
| 2.5 or 5 μM repair oligonucleotide | 0.25 μM | c |
|
| ||
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