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(A) Monitoring of extracellular GFP fluorescence intensity and cell viability of P. aeruginosa GFP over time after treatment with carvacrol at low concentration. (B) Epifluorescence microscopy visualization of P. aeruginosa GFP cells after staining with SYTO-9 (green fluorescence for living bacteria) and propidium iodide (red fluorescence for dead bacteria). Data are presented as the means (±SD) of three independent experiments. Control represents cells treated with HEPES buffer.
