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. 2022 Jun 21;18(6):e1010269. doi: 10.1371/journal.pgen.1010269

Fig 3. MLLE3Rrm4 is crucial for PAM2L1Upa1 and PAM2L2Upa1 binding.

Fig 3

(A) Schematic representation of protein variants (Molecular weight in kilo Dalton indicated) using the following colouring: dark green, RNA recognition motif (RRM); orange, MLLERrm4 domains; dark blue, MLLEPab1; light blue PAM2; light orange PAM2L sequence (PL1–2). Ankyrin repeats (5xANK), FYVE domain, and RING domain of Upa1 are given in dark grey. GST and SUMO tags are labelled. Variant amino acids of the FxP and FxxP of PAM2 and PAM2L sequences are printed in grey font. (B-D) Representative isothermal titration calorimetry (ITC) binding curves of MLLE domains. Experiments were performed using GST- or Histidine-tagged MLLE variants and synthetic PAM2 peptide variants. KD values of two independent measurements are given (values corresponding to the indicated data are given in bold).