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. 2022 Jun 13;18(6):e1010620. doi: 10.1371/journal.ppat.1010620

Fig 8. Bile acid (BA) treatment alters the trafficking dynamics of SADS-CoV along the endo-lysosomal system.

Fig 8

(A) Porcine intestinal enteroid (PIE) monolayers treated with medium alone or 100 μM cholic acid (CA) for 1 h. Images were acquired by confocal laser-scanning microscopy, detecting Rab5, Rab7 and LAMP1 (green), and nuclei (blue) were visualized by DAPI (scale bar, 10 μm). (B) PIE monolayers were infected with R18-SADS-CoV (red) at an MOI = 20 in the presence or absence of 100 μM CA and incubated at 37°C for 5, 15, 30 or 60 min. The cells were immunostained with Rab5, Rab7, or LAMP1 (green), and nuclei (blue) were visualized by DAPI (scale bar, 5 μm). Images were collected on an LSM880 confocal laser-scanning microscope (Zeiss). Pearson’s correlation coefficient analysis was carried out using Image J software. PIE monolayers were pretreated with cathepsin inhibitor (C) E-64 or (D) teicoplanin for 2 h, and then inhibitors and CA were added to the medium at the indicated concentrations during and after SADS-CoV-GFP infection for 48 h.