Figure 5.

HIF-1α was identified as a target miR-155 gene as follows. (a) Sequence alignment of binding site between HIF-1α and miR-155. (b) The experiments of the dual-luciferase reporter gene detected the fluorescence activity of HIF-1α-wt/mut under the treatment of miR-155/NC mimics, ^∗∗∗p < 0.001 against a mimic group of HIF-1α-wt + NC. (c) Western blot measured the protein expression of HIF-1α level. (d) The expression of mRNA of HIF-1α after transfection of miR-155 mimics/inhibitor was performed by RT-qPCR. ^∗∗p < 0.001 against a group of NC mimics and a group of NC inhibitor vs. ^##p < 0.01.