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. 2022 Jun 24;2022:1157498. doi: 10.1155/2022/1157498

Figure 2.

Figure 2

Effect of hUCMSC-EVs on the proliferation, migration, and cartilage differentiation of BMSCs. BMSCs were treated with hUCMSC-CM alone or combined with hUCMSC-GW4869-CM. (a) Proliferation of BMSCs was detected by the CCK8 assay. (b) Migration of BMSCs was detected by the Transwell assay (scale bar = 50 μm). (c) Cartilage differentiation ability of BMSCs was detected by Alcian blue staining (scale bar = 50 μm). (d) mRNA levels of collagen II, aggrecan, and Sox9 in BMSCs were determined by RT-qPCR. (e) Protein levels of collagen II, aggrecan, and Sox9 in BMSCs were measured by Western blot analysis. ∗p < 0.05, compared with the control; #p < 0.05, compared with BMSCs treated with hUCMSC-CM. All experiments were repeated for three times.