Skip to main content
. 2022 Jun 9;25(7):104567. doi: 10.1016/j.isci.2022.104567

Figure 2.

Figure 2

Histological analysis of pancreatic α-cell number and β-cell number in the pancreatic tissues of two T2D mouse models treated with GCGR mAb or IgG control for 4 weeks

(A and C) Representative image of an islet immunostained for glucagon and insulin in db/db mice (A) and HFD + STZ-induced T2D mice (C) treated with GCGR mAb or IgG control. The arrows indicate co-labeled cells. The cells in the small box are enlarged at the right of the image. Scale bar = 50 μm.

(B and D) Quantification of the number of α-cells and β-cells per islet slice, and the ratio of β-cell number and α-cell number in db/db mice (B) and HFD + STZ-induced T2D mice (D).n = 3-5 sections/mouse multiplied by 6 mice/group in db/db mice, and n = 3-5 sections/mouse multiplied by 9 mice/group in HFD + STZ-induced T2D mice. Data are expressed as the median (interquartile range). Statistical analysis was conducted by the Mann-Whitney test. ∗∗p < 0.01, ∗∗∗p < 0.001 vs. control; §p < 0.05, §§p < 0.01 vs. db/m. See also Figure S2.