Figure 3.

Immunofluorescent analysis of progenitor-derived β-cell neogenesis in the pancreatic tissues of T2D mice treated with GCGR mAb or IgG control for 4 weeks

(A and B) Representative photograph showing glucagon⁺ cells and insulin⁺ cells located in the ductal region of db/db mice (A) and HFD + STZ-induced T2D mice (B). The arrows indicate glucagon⁺ cells or insulin⁺ cells in the ductal lining.

(C) Representative image of an islet immunostained with RFP (Ngn3⁺ cell lineage-tracing marker) and insulin in Ngn3⁺ cell lineage-tracing T2D mice that were induced by HFD + STZ. The arrows indicate co-labeled cells, and the ductal lumen is outlined with dashed lines. The cells in the small box are enlarged at the right of the image. Scale bar = 50 μm. See also Figures S3 and S4.