Figure 5.

Immunofluorescent analysis of α-to-β cell conversion in the pancreatic tissues of T2D mouse models treated with GCGR mAb or IgG control for 4 weeks

(A and B) Quantification of glucagon⁺insulin⁺ cells in db/db mice (A) and HFD + STZ-induced T2D mice (B) as shown in (Figures 2A and 2C).

(C and D) Representative image of an islet immunostained with β-gal (α-cell lineage-tracing marker) and insulin (C), and quantification of β-gal⁺insulin⁺ cells (D) in α-cell lineage-tracing T2D mice that were induced by HFD + STZ. The arrows indicate co-labeled cells. The cells in the small box are enlarged at the right of the image. Scale bar = 50 μm. n = 5 sections/mouse multiplied by 6 mice/group in db/db mice, n = 3 sections/mouse multiplied by 9 mice/group in HFD + STZ-induced T2D mice, and n = 3 sections/mouse multiplied by 5 mice/group in the α-cell lineage-tracing T2D mice. Data represent the mean ± SEM. Statistical analysis was conducted by Student’s t-test. ∗∗∗p < 0.001 vs. control. See also Figures S5, S6, and S7.