Figure 6.

Hormone production and gene expression in primary mouse islets incubated with GCGR mAb (1,000 nmol/L) or IgG (as control) for 24 h in high glucose (25 mmol/L) medium

(A–D) Intracellular glucagon content (A), supernatant glucagon level (B), intracellular insulin content (C), and supernatant insulin level (D) were detected by ELISA. The hormone levels were normalized to total protein content.

(E) Glucose-stimulated insulin secretion assay in the islets after treated with GCGR mAb or IgG. The insulin levels were normalized to total protein content.

(F) Gene expression was determined by quantitative RT-PCR. The experiments were repeated 3 times. Data represent the mean ± SEM. Statistical analysis was conducted by Student’s t-test or two-way ANOVA as appropriate. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. control. See also Figure S8.