Antibodies |
|
Anti-MITOL rabbit polyclonal antibody |
In house |
N/A |
Anti-FLAG-M2 |
Sigma |
F-1804; RRID: AB_262044
|
Anti-αActinin |
Sigma |
A-7732; RRID: AB_2221571
|
Anti α-tubulin |
Sigma |
T-9026; RRID: AB_477593
|
Anti-HA antibody |
MBL |
M132-3; RRID: AB_10207271
|
Antibodies against Drp1 |
BD Biosciences |
#611112; RRID: AB_398423
|
Antibodies against Tom20 |
BD Biosciences |
#612278; RRID: AB_399595
|
Anti-GFP antibody |
MBL |
M048-3; RRID: AB_591823
|
Anti-NDUFA9 antibody |
Invitrogen |
#459100; RRID: AB_2532223
|
Anti-COX4 |
Invitrogen |
PA5-29992; RRID: AB_2547466
|
Anti-Troponin T |
Abcam |
ab8295; RRID: AB_306445
|
Anti-Drp1 |
Abcam |
ab56788; RRID: AB_941306
|
Anti-ATP5A |
Abcam |
ab14748; RRID: AB_301447
|
Anti-UQCRC2 |
Abcam |
ab14745; RRID: AB_2213640
|
Anti-VDAC |
Cell Signaling |
#4866; RRID: AB_2272627
|
Anti-Caspase-3 |
Cell Signaling |
#9662; RRID: AB_331439
|
Anti-cleaved Caspase3 |
Cell Signaling |
#9661; RRID: AB_2341188
|
Anti-SDHA |
Cell Signaling |
11998S; RRID: AB_2750900
|
Mitochondrial Marker Antibody Sampler Kit |
Cell Signaling |
#8674; RRID: AB_11217817
|
Mitochondrial Dynamics Antibody Sampler Kit II |
Cell Signaling |
#74792 |
Anti-GAPDH |
Acris Antibodies |
ACR001-PT; RRID: AB_1616730
|
|
Biological samples |
|
Human adult normal heart tissue lysate |
BioChain |
P1234138 |
Human adult normal heart tissue lysate |
BioChain |
P1234122 |
Human adult infarct tissue lysate |
BioChain |
P1236138Hd-1 |
Human adult infarct tissue lysate |
BioChain |
P1236122Hd-1 |
|
Chemicals, peptides, and recombinant proteins |
|
4-OHT |
Sigma |
H7904 |
Tamoxifen |
Cayman Chemical |
#13258 |
Isoproterenol |
nacalai tesque |
#19703-04 |
Digitonin |
WAKO |
#044-02121 |
|
Critical commercial assays |
|
RNeasy kit |
QIAGEN |
#74134 |
QuantiTect Reverse Transcription Kit |
QIAGEN |
#205311 |
THUNDERBIRD SYBR qPCR Mix |
TOYOBO |
QPS-201 |
Masson’s trichrome staining |
Sigma |
HT15-1KT |
Senescence β-Galactosidase Staining Kit |
Cell Signaling |
#9860S |
Protein carbonyl kit |
SHIMA Laboratories |
#ROIK03 |
luciferase-based ATP determination kit |
TOYO B NET |
TA100 |
In Situ Cell Death Detection Kit |
Roche |
11684795910 |
|
Experimental models: Cell lines |
|
MITOLflox/flox MEFs |
Sugiura et al. (2013) |
N/A |
|
Experimental models: Organisms/strains |
|
Mouse: MITOLflox/flox
|
Sugiura et al. (2013) |
N/A |
Mouse: αMHC-MerCreMer |
Sohal et al. (2001) |
N/A |
Rat: Slc:Wistar |
SLC |
N/A |
|
Oligonucleotides |
|
All qPCR primer sequences listed in Table S1 in Method details. |
|
|
|
Recombinant DNA |
|
pAAV/cTnT-GFP-P2A-MITOL-WPRE |
This paper |
N/A |
pAAV/cTnT-GFP-P2A-K38A Drp1-WPRE |
This paper |
N/A |
|
Software and algorithms |
|
Fiji |
ImageJ |
https://imagej.net/software/fiji/downloads |
Prism |
GraphPad |
https://www.graphpad.com/ |
Adobe Photoshop |
Adobe |
https://www.adobe.com/es/ |
Adobe Illustrator |
Adobe |
https://www.adobe.com/es/ |
ImageJ |
ImageJ |
https://imagej.nih.gov/ij/ |