REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-MITOL rabbit polyclonal antibody	In house	N/A
Anti-FLAG-M2	Sigma	F-1804; RRID: AB_262044
Anti-αActinin	Sigma	A-7732; RRID: AB_2221571
Anti α-tubulin	Sigma	T-9026; RRID: AB_477593
Anti-HA antibody	MBL	M132-3; RRID: AB_10207271
Antibodies against Drp1	BD Biosciences	#611112; RRID: AB_398423
Antibodies against Tom20	BD Biosciences	#612278; RRID: AB_399595
Anti-GFP antibody	MBL	M048-3; RRID: AB_591823
Anti-NDUFA9 antibody	Invitrogen	#459100; RRID: AB_2532223
Anti-COX4	Invitrogen	PA5-29992; RRID: AB_2547466
Anti-Troponin T	Abcam	ab8295; RRID: AB_306445
Anti-Drp1	Abcam	ab56788; RRID: AB_941306
Anti-ATP5A	Abcam	ab14748; RRID: AB_301447
Anti-UQCRC2	Abcam	ab14745; RRID: AB_2213640
Anti-VDAC	Cell Signaling	#4866; RRID: AB_2272627
Anti-Caspase-3	Cell Signaling	#9662; RRID: AB_331439
Anti-cleaved Caspase3	Cell Signaling	#9661; RRID: AB_2341188
Anti-SDHA	Cell Signaling	11998S; RRID: AB_2750900
Mitochondrial Marker Antibody Sampler Kit	Cell Signaling	#8674; RRID: AB_11217817
Mitochondrial Dynamics Antibody Sampler Kit II	Cell Signaling	#74792
Anti-GAPDH	Acris Antibodies	ACR001-PT; RRID: AB_1616730
Biological samples
Human adult normal heart tissue lysate	BioChain	P1234138
Human adult normal heart tissue lysate	BioChain	P1234122
Human adult infarct tissue lysate	BioChain	P1236138Hd-1
Human adult infarct tissue lysate	BioChain	P1236122Hd-1
Chemicals, peptides, and recombinant proteins
4-OHT	Sigma	H7904
Tamoxifen	Cayman Chemical	#13258
Isoproterenol	nacalai tesque	#19703-04
Digitonin	WAKO	#044-02121
Critical commercial assays
RNeasy kit	QIAGEN	#74134
QuantiTect Reverse Transcription Kit	QIAGEN	#205311
THUNDERBIRD SYBR qPCR Mix	TOYOBO	QPS-201
Masson’s trichrome staining	Sigma	HT15-1KT
Senescence β-Galactosidase Staining Kit	Cell Signaling	#9860S
Protein carbonyl kit	SHIMA Laboratories	#ROIK03
luciferase-based ATP determination kit	TOYO B NET	TA100
In Situ Cell Death Detection Kit	Roche	11684795910
Experimental models: Cell lines
MITOLflox/flox MEFs	Sugiura et al. (2013)	N/A
Experimental models: Organisms/strains
Mouse: MITOLflox/flox	Sugiura et al. (2013)	N/A
Mouse: αMHC-MerCreMer	Sohal et al. (2001)	N/A
Rat: Slc:Wistar	SLC	N/A
Oligonucleotides
All qPCR primer sequences listed in Table S1 in Method details.
Recombinant DNA
pAAV/cTnT-GFP-P2A-MITOL-WPRE	This paper	N/A
pAAV/cTnT-GFP-P2A-K38A Drp1-WPRE	This paper	N/A
Software and algorithms
Fiji	ImageJ	https://imagej.net/software/fiji/downloads
Prism	GraphPad	https://www.graphpad.com/
Adobe Photoshop	Adobe	https://www.adobe.com/es/
Adobe Illustrator	Adobe	https://www.adobe.com/es/
ImageJ	ImageJ	https://imagej.nih.gov/ij/