Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 1;13:3799. doi: 10.1038/s41467-022-31135-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — Ldlr^−/− and T-Abc^dkoLdlr^−/− mice were fed a chow diet for 12–13 months. Hearts were isolated and paraffin sections of the aortic root were stained for (a) H&E or (b, c) CD3. a Representative pictures of H&E staining (left) and quantification (right) of atherosclerotic lesion area (p = 0.003). Scale bar represents 200 μm. n = 11 Ldlr^−/− and n = 10 T-Abc^dkoLdlr^−/− mice. b Representative pictures of CD3 staining on atherosclerotic lesions (left) and quantification (right) of total CD3⁺ cells per section (p = 0.008). T cells were identified as cells with brown plasma membrane CD3 staining and the hematoxylin staining still visible. CD3⁺ cells are depicted by arrows. Scale bar represents 80 μm. c CD3⁺ T cells as percentage of total cells per section (p = 0.0107). b, c n = 11 Ldlr^−/− and n = 10 T-Abc^dkoLdlr^−/− mice. d–i Aortas were collected. d Aortic CD3⁺ T cells were isolated, RNA was extracted, and Foxp3, Il10, Tgfb1, tumor necrosis factor (Tnf), Bcl2 (p = 0.023), and Cdkn1a (p = 0.025) mRNA expression were measured by qPCR. n = 7 Ldlr^−/− and n = 5 T-Abc^dkoLdlr^−/− mice. e Total aortic cells were labeled with CFSE, stimulated with αCD3/αCD28 beads, and stained for CD4 after 96 h of stimulation. CFSE dilution was measured by flow cytometry and the number of divisions were quantified for aortic CD4⁺ T cells. n = 8 Ldlr^−/− and n = 8 T-Abc^dkoLdlr^−/− mice. f–i Total aortic cells were stained for CD4, CD44, CD62L, and cleaved caspase 3/7. f Representative flow cytometry plots of cleaved caspase 3/7 in CD4⁺ T cells gated as in Supplementary Fig. 12d, and (g) quantification (p = 0.015). n = 4 Ldlr^−/− and n = 5 T-Abc^dkoLdlr^−/− mice. h Aortic CD4⁺ T_mem/eff cells (p = 0.0003) as percentage of aortic CD4⁺ T cells and (i) cleaved caspase3/7 in aortic CD4⁺ T_mem/eff cells (p = 0.033). n = 7 Ldlr^−/− and n = 7 T-Abc^dkoLdlr^−/− mice. For all panels, error bars represent SEM. Biologically independent samples were included. p value was determined by unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.