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. 2022 Jul 1;5:648. doi: 10.1038/s42003-022-03614-3

Fig. 4. FGF2 induces Survivin expression and enriches Survivin in exosomes.

Fig. 4

a Survivin mRNA and protein abundance in E8.5 wild type (WT) and FGF2 Tg embryos from nondiabetic or diabetic dams. b Luciferase reporter activity driven by the Survivin promoter. Re-FGF2: human recombinant FGF2. c Survivin mRNA and protein expression in normal glucose (NG; 5 mM glucose) and high glucose (HG; 25 mM glucose) culture conditions with Re-FGF2. d Luciferase reporter activity driven by the Survivin promoter. si-FGF2: siRNA FGF2. e Survivin mRNA and protein expression. f, g Exosome number and Survivin protein expression in exosomes treated by Re-FGF2 (f) or si-FGF2 (g). h, i Exosome number and Survivin protein expression in exosomes of isolated E8 Flk-1+ progenitors treated by Re-FGF2 (h) or si-FGF2 (i). In (f, h), the vehicle control is water (equal volume of Re-FGF2 solution). Cell culture experiments were performed three times (n = 3). Cells experiments were repeated three times (n = 3). Experiments were performed using three embryos from three different dam per group (n = 3). * indicates significant difference (P < 0.05) compared to other groups (P < 0.05). ND nondiabetic, DM diabetes mellitus, WT wild type, FGF2 FGF2 transgenic mice.