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. 2022 Jul 1;5:648. doi: 10.1038/s42003-022-03614-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a GFP was robustly expressed in Flk-1⁺ progenitors in the yolk sacs of E8.5 conceptuses. Bars = 200 μm. Survivin mRNA (b) and protein (c) expression in E8.5 embryos. d Imaging and quantification of Flk-1⁺ progenitors in the yolk sacs of E8.5 conceptuses. Cell nuclei were stained with DAPI (blue). Bars = 100 μm. e Imaging and quantification of blood island numbers in the yolk sacs of E8.5 conceptuses (HE staining). Bars = 100 μm. f Blood vessel density determined by CD31 staining in the yolk sacs of E8.5 conceptuses. Bars = 25 μm. g View of blood vessels in the yolk sacs of E8.5 conceptuses. Bars = 100 μm. h CD31 staining in E8.5 embryos showing disruptive blood vessel formation in embryos exposed to maternal diabetes. Bars = 125 μm. Experiments were performed using three embryos from three different dam per group (n = 3). * indicates significant difference compared to other groups (P < 0.05). ND nondiabetic, DM diabetes mellitus, WT wild type, Survivin Survivin transgenic mice.