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. 2022 Jul 1;5:648. doi: 10.1038/s42003-022-03614-3

Fig. 6. Restoring Survivin expression in Flk-1+ progenitors reduces diabetes-induced apoptosis and NTD formation.

Fig. 6

a Protein levels of cleaved caspase 3 and caspase 8 in E8.5 embryos. b Representative TUNEL assay imaging showing apoptotic cells (red dots) in the E8.5 neuroepithelium and quantification of TUNEL-positive cell numbers in the bar graph. Cell nuclei were stained with DAPI (blue), bars = 30 μm. c Representative imaging of WT and Survivin transgenic overexpressing embryos showing an NTD embryo with an open neural tube. Bars = 200 μm and 100 μm, for the top panel and the low panel, respectively. Experiments were performed using three embryos from three different dam per group (n = 3). * indicates significant difference compared to other groups (P < 0.05). ND nondiabetic, DM diabetes mellitus, WT wild type, Survivin Survivin transgenic overexpressing embryos.