Fig. 6. IL-9 regulates the transcriptional profile of IMs.

a–f RNA-Seq analysis on macrophage populations isolated by flow-sorting from intact lungs of B16 tumor-bearing mice. a, b Differentially expressed gene between WT and Il9r^−/− macrophages. c GSEA analysis on the differentially expressed gene between WT and Il9r^−/− CD11c⁺ IMs. d Heatmap showing gene expression related to cancer module 333. e Pathway analysis on differentially expressed genes in WT and Il9r^−/− IMs. f Heatmap showing gene expression in WT and Il9r^−/− macrophage populations. g Immunofluorescence analysis of CD31⁺ cells of lung sections from tumor bearing mice, Scale bar = 10 µm. h Il9r^−/− mice were injected with B16 tumor cells on day 0, PBS/WT IMs were transferred to recipient mice on day 14. Lung CD31⁺ cells were analyzed by flow on day 21 (n = 4 mice for Il9r^−/− group, n = 6 mice for Il9r^−/− + CD11c⁺ IM group). i Venn diagram showing the overlap of differentially expressed genes in CD11c⁺ IMs and CD11c⁻ IMs. j Heatmap showing the expression of selected genes common between the IM populations. k Venn diagram showing common genes between IM specific genes and differentially expressed (DE) genes regulated by Il9r-deficiency in IMs. Data are the mean ± SEM. Unpaired two-tailed Student t-test was used for comparison in h.