FIG. 4.

Transformation of DMS by culture components of strain DMS-S1. The strain was grown in NSYE medium with 20 μM Na₂SO₄, and the culture medium was separated into components as described in the text. Forty-five micromoles of DMS was added to 4.5 ml of culture component solution in 22-ml vials (7.8 mM DMS in the solution). (A) Whole culture medium containing cells of strain DMS-S1 (○), culture supernatant (□), and cells in buffer (●). (B) Whole culture medium containing cells heated for 5 min at 105°C (◊) and culture supernatant heated for 5 min at 105°C (⧫). (C) Components of culture supernatant whose molecular weight was higher than 10,000 in buffer (▴) and culture supernatant after exclusion of the components with a molecular weight higher than 10,000 (▵).