TABLE 2.
Accumulation of formaldehyde and formate by culture components of strain DMS-S1a
| Culture component(s) | Heat treatmentb | Added DMS | Amt (μmol) of accumulated:
|
|
|---|---|---|---|---|
| Formaldehyde | Formate | |||
| Cells and supernatant | − | − | 0.01 | 0.29 |
| + | 0.21 | 1.51 | ||
| + | − | 0.14 | 0.35 | |
| + | 0.37 | 0.47 | ||
| Cells in NS buffer | − | − | 0.03 | 0.10 |
| + | 0.12 | 0.24 | ||
| + | − | 0.05 | 0.02 | |
| + | 0.09 | 0.08 | ||
| Supernatant | − | − | 0.10 | 0.28 |
| + | 0.25 | 0.28 | ||
| + | − | 0.15 | 0.37 | |
| + | 0.44 | 0.53 | ||
a
Strain DMS-S1 was grown on 0.2% (34 mM) ethanol with 10 μM Na2SO4 for 6 days. Then the culture was separated into culture components, and 40 μmol of DMS was added to 20 ml of culture component solution in 71-ml test tubes. After cultivation for 11 to 17 days, formate and formaldehyde in a pair of culture component solutions were quantified. Mean values are shown.
b
Samples were heated for 5 min at 105°C.