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. 2022 Jul 1;12:11190. doi: 10.1038/s41598-022-15524-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EGFP and NIS proteins in transiently and stably expressing cancer cells. (A) EGFP fluorescence in Huh7 cells transduced with NIS/EGFP adenovirus (Huh7/NIS-Adv cells). (B) Western blots of NIS protein obtained from the cell membrane fraction (top) and whole cell lysates (bottom) of Huh7, Huh7/NIS-Adv, MDAMB231, and MDAMB/NIS-stable cancer cells. (C) ¹²⁵I uptake (right) in Huh7/NIS-Adv and MDAMB/NIS-stable cells compared to respective Huh7 and MDAMB control cells. Bars for uptake are mean ± SD of uptake in fmol/mg protein obtained from triplicate samples per group. WT wild type. ^‡P < 0.0001.