Table 2.

PBD experimental runs for screening the significant independent factors affecting the growth of CW-S.

Run Order	A	B	C	D	E	F	G	H	Response
	Agitation	Aeration	Inoculum Size	Carbon Source percentage	Nitrogen Source percentage	Incubation Time	pH	Temp	Cell concentration (× 108 CFU/mL)
1	− 1	− 1	− 1	− 1	− 1	− 1	− 1	− 1	2.54 ± 0.09
2	1	1	− 1	1	1	− 1	1	− 1	2.94 ± 0.03
3	1	− 1	− 1	− 1	1	1	1	− 1	4.82 ± 0.04
4	− 1	− 1	− 1	1	1	1	− 1	1	5.64 ± 0.07
5	1	1	− 1	1	− 1	− 1	− 1	1	2.80 ± 0.04
6	1	− 1	1	− 1	− 1	− 1	1	1	5.10 ± 0.04
7	− 1	1	1	1	− 1	1	1	− 1	5.14 ± 0.02
8	− 1	− 1	1	1	1	− 1	1	1	6.62 ± 0.05
9	− 1	1	− 1	− 1	− 1	1	1	1	7.68 ± 0.07
10	1	− 1	1	1	− 1	1	− 1	− 1	3.42 ± 0.06
11	− 1	1	1	− 1	1	− 1	− 1	− 1	2.84 ± 0.03
12	1	1	1	− 1	1	1	− 1	1	5.52 ± 0.17

Within columns, CFU ± SD values (n = 3).