Table 4.

ANOVA for the CCD quadratic model.

Analysis of variance table [partial sum of squares—type iii]
SOURCE	Sum of Squares	df	Mean Square	F value	p value	Significance
Model	92.23	14	6.59	539.72	< 0.0001	Significant
A-Temperature	0.5251	1	0.5251	43.02	< 0.0001
B-Incubation time	0.2501	1	0.2501	20.49	0.0004
C-pH	2.58	1	2.58	211.43	< 0.0001
D-Agitation	0.1001	1	0.1001	8.20	0.0118
AB	0.0163	1	0.0163	1.33	0.2665
AC	0.1243	1	0.1243	10.18	0.0061
AD	1.27	1	1.27	104.15	< 0.0001
BC	0.2525	1	0.2525	20.69	0.0004
BD	0.6683	1	0.6683	54.75	< 0.0001
CD	0.2328	1	0.2328	19.07	0.0006
A2	68.59	1	68.59	5619.33	< 0.0001
B2	2.00	1	2.00	163.94	< 0.0001
C2	6.59	1	6.59	539.76	< 0.0001
D2	27.30	1	27.30	2236.36	< 0.0001
Residual	0.1831	15	0.0122
Lack of fit	0.1449	10	0.0145	1.90	0.2481	Not significant
Pure error	0.0382	5	0.0076
Cor total	92.41	29

STD. DEV. 0.1105, MEAN 7.10, C.V. % 1.56.

R² 0.9980, ADJUSTED R² 0.9962, PREDICTED R² 0.9904, ADEQ PRECISION 84.7552.