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High and low levels of factors used in PBD.
| S/N | Coded factors | Factors | Low level (−1) | High level (+ 1) |
|---|---|---|---|---|
| 1 | A | Agitation speed (rpm) | 150 | 300 |
| 2 | B | Aeration (L/h) | 8 | 10 |
| 3 | C | Inoculation Percentage (v/v ;%) | 2 | 3 |
| 4 | D | Carbon source percentage (Molasses; (w/v ;%) | 2 | 3 |
| 5 | E | Nitrogen source percentage (Urea; w/v ;%) | 1.5 | 3 |
| 6 | F | Incubation time (hours) | 24 | 36 |
| 7 | G | pH | 6 | 8 |
| 8 | H | Temperature (°C) | 28 | 40 |
