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Actual and coded values of the factors in employment in the CCD.
| Factors | Range of Levels | |||||
|---|---|---|---|---|---|---|
| Code | − 2 | − 1 | 0 | 1 | 2 | |
| Temperature (°C) | A | 25 | 30 | 35 | 40 | 45 |
| Incubation Time (hours) | B | 24 | 28 | 32 | 36 | 40 |
| pH | C | 6.5 | 7 | 7.5 | 8 | 8.5 |
| Agitation (rpm) | D | 150 | 200 | 250 | 300 | 350 |
