Figure 4.

Epithelial Smyd5 ablation does not cause basal changes in the numbers of stem or goblet cells. (A) Representative images of IF staining of BMI1 (green; left) and quantitative analysis (right) of BMI1-positive cells per crypt in colon tissues from Smyd5^fl/fl and Smyd5^ΔIEC mice. Nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). n = 5 per group. (B) Western blot analysis of LGR5 expression in primary IECs isolated from Smyd5^fl/fl (n = 4) and Smyd5^ΔIEC mice (n = 5). (C) Representative IHC staining (left) and quantitative analysis (right) for KLF5 in colon tissues from Smyd5^fl/fl and Smyd5^ΔIEC mice. n = 9 per group. (D) Representative images of PAS staining (left) and quantitative analysis (right) of PAS-positive cells per crypt in colon tissues from Smyd5^fl/fl and Smyd5^ΔIEC mice. n = 5 per group.