Table 1.
Primer Sequences for Genotyping
| Primer | Sequence 5’ → 3’ | Primer type | Note |
|---|---|---|---|
| Primers for Smyd5 genotyping | |||
| Smyd5_F | GGTCTCATGGGGAACTGAGG | WT and mutant forward | Designed by EMMA (European Mouse Mutant Archive) |
| Smyd5_R | GCTTTCAGCCAAGCCAAGTC | WT reverse | |
| CAS_R1_Term | TCGTGGTATCGTTATGCGCC | Mutant reverse | |
| Primers for Vil1-Cre genotyping | |||
| 18960 | TTCTCCTCTAGGCTCGTCCA | Transgene reverse | Designed by The Jackson Laboratory |
| 14506 | CATGTCCATCAGGTTCTTGC | Transgene forward | |
| oIMR7338 | CTAGGCCACAGAATTGAAAGATCT | Internal positive control forward | |
| oIMR7339 | GTAGGTGGAAATTCTAGCATCATCC | Internal positive control reverse | |