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. 2022 Jul 1;5:646. doi: 10.1038/s42003-022-03582-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Differential expression analysis by RNA seq in 293T cells following HGF WT and HGF 4Cys-4Ala treatment as indicated confirms that core cysteines in HGF PAN domain are necessary for the expression of a wide range of genes. Responsive genes were normalized to FPKM value for non-treated cells and then normalized to HGF WT treated cells. Data represents the average of three independent biological replicates and *p < 0.05, **p < 0.005 and ***p < 0.0005 were calculated with a student’s t-test.