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. 2022 Jun 24;3(3):101507. doi: 10.1016/j.xpro.2022.101507

Figure 2.

Figure 2

Immunostaining

(A) Fixed OTCS are best removed by carefully scraping them from the Millipore insert with a metal spatula (step 4).

(B) The quality of immunostaining prior to expansion microscopy can be assessed by placing an OTC on a slide, drawing a closed circle around it with a PAP pencil, and carefully adding enough PBS to prevent a staining background caused by drying and to allow imaging with a water-dipping objective (step 10).

(C) Representative image of a successful immunostaining for GFP (green) and GluA2 (red). This is a single step within a Z-stack to better visualize GluA2 staining. Scale bar: 5 μm.